Syllabus – Junior Scientific Officer @HPPSC,Shimla

Syllabus:-


The CBTs/ Offline objective type screening test shall be of two hour’s duration consisting of 100 Multiple Choice
Questions (MCQ) from the syllabus as detailed below:-
A. Common Syllabus:- (45 Marks)
Definition and sources of pollution, Different types of pollution, Environmental segments. Composition of
the atmosphere, Chemical composition of Air, Chemical and photochemical reactions in the atmosphere,
Types and sources of water, air & soil pollution, Oxides of C,N,S and their effects on flora and fauna, Acidrain, Photochemical smog formation, Green house effects (global warming and ozone depletion), Introduction to analytical methods for monitoring and analysis of water, air and soil pollution, Noise
pollution, Soil composition, Physical and chemical characteristics, Micro and macro nutrients, Soil
pollution-fertilizers and pesticides, Bio-remediation and bio-restoration of contaminated soils.
Hydrological cycle, Water pollution: inorganic, organic, pesticides, industrial and radioactive materials, oil
spills and oil pollutants, Eutrophication and its impact on organisms and communities, Impact of heavy
metals, halogens, radio-nucleotides on aquatic flora and fauna, Aerobic treatment of waste water (Trickling
Filters, Rotating Biological Contractors, Fluidized bed reactors, Packed bed reactors), Anaerobic Baffled
Digesters, Up flow Anaerobic Sludge blanket reactors, and Industrial waste water treatment, Advanced
waste water treatment for removal of major pollutants, Emerging biotechnological and Nano-technological
processes in waste water treatment. Chemical kinetics, Alcohols, Phenols, Ether, Aldehydes and Ketones, Basic principles of thermodynamics,
Chemical & lonic Equilibrium, Solutions and Electrochemistry, Carbohydrates, Periodic properties of
elements, Acid and Bases, Basic concepts of organic reactions, Redox Reactions. Basic Principles of spectroscopy, Principle, working and applications of UV, Visible IR, H-NMR for the determination of structure of simple organic compounds, Spectrophotometer, Flame Photometer, Atomic
Absorption Spectrophotometer, GC, GC-MS, HPLC, Chromatography (Paper, TLC, Gel Filtration Ionexchange Chromatography, Reverse phase chromatography, Hydrophobic interactions, Affinity chromatography, X-ray Diffraction, Microscopy, Electron Microscopy: SEM, TEM, AFM, Electrophoresis,
Centrifugation. Statistical analysis of data, Types and source of errors, Propagation of errors, Detection and minimization
of various types of errors, Uncertainty, Accuracy and precision, Average and standard deviation, Variance
and confidence interval, Tests of significance (F-test, t-test and paired t-test), Criteria for the rejection of
analytical data (4d rule, 2.5 d rule, Q-test, average deviation and standard deviation), Least-square
analysis. Overview of metabolic processes (catabolic and anabolic), Energy transfer processes, Role and
significance of ATP (the biological energy currency), Glycolysis, TCA Cycle, Pentose Phosphate Pathway,
Gluco-genesis, ETC and oxidative phosphorylation, Metabolism of proteins and lipids, their structure,
nomenclature and properties, Biosynthesis of proteins and glycerides, Acyl-glycerol, Phospho-glycerides,
Sphingolipids, Glycosphingolipids Catabolism of fatty acids. Amino acids as building block of proteins, their structure and classification and chemical properties, Simple peptides, Structure of peptide bond, Organizational levels of protein structure, Properties of proteins:
simple, conjugated, fibrous and globular proteins, Purine and pyrimidine bases of nucleic acids, Base
pairing via H-bonding. Structure of ribonucleic acids (RNA) and deoxyribonucleric acids (DNA), Double
helix model of DNA and forces responsible for holding it, Chemical and enzymatic hydrolysis of nucleic
acids, Replication of DNA, Transcription, Translation and genetic code, Chemical synthesis of mono and
tri-nucleoside, Structure, Properties and classification of Porphyrins, Biosynthesis and degradation
enzymes: nature, co-enzymes, cofactors and prosthetic groups, classification, factors affecting enzyme
activity. Page 8 of 14
B. Subject Specific Syllabus: (25 Marks)
B.1 Chemistry:
Molecular theory of hetero diatomic molecules, Band theory of bonding in metals, Hydrogen bonding,
Solid state chemistry: radius ratio rule, Space lattice (only cubes), Type of unit cell, Bragg’s Law,
Calculation of density of unit cell, One and two Dimensional solids, Graphite as two dimensional solid
and its conducting properties, Fullerene and its applications, Molecular structure and basic quantum
chemistry, Schrodinger equation, Interpretation of wave function, Hydrogen atom, Atomic and
molecular orbitals, Phase Rule, Equilibrium potential, Electrochemical cells (galvanic and
concentration cells), Electrochemical theory of corrosion and protection of corrosion, Surfactants
(cationic, anionic and non-ionic detergents), Polymers, Stability of reaction intermediates e.g.
carbanions, carbocations and free radicals, Types of organic reactions and mechanism of nucleopile
substitution reactions, Common organic reactions and their mechanis, Stability of cycloalkanes,
Resonance concept, Inductive and mesomeric effects, Directive effects, Activating and deactivating
group, Hydrogen bonding, Reagents in organic synthesis of selected organic name reactions and their
mechanisms, Nucleophilic substitution reaction, Eletrophilic and free radical addition reactions,
Electrophilic aromatic substitutions reactions, Nucleophilic addition (principles of nucleophilic addition
to carbonyl groups, reduction & oxidation), Elimination reactions, E-Z and R, S Nomenclature, Optical
isomerism of organic compounds containing one chiral centre, Examples of optically active compounds
without chirality, Conformations of n-butane, Concept of aromaticity, properties of conjugated systems,
Group theory, Non-aqueous solvents, Inorganic hydrides, Pericyclic reactions.
B.2 Environmental Science:-
Environmental geoscience and geology, Geomorphological processes, Environmental geochemistry,
Concept and scope of ecology and biological diversity, Ecosystem concepts and dynamics, Study of
different ecosystems, Disaster mitigation and management, Environmental laws, policies and
regulations, Environmental treaties and conventions, Natural resources-laws, conservation strategy
and management, Thermal and radiation pollution, Concept of ecotoxicology and radiation impacts,
Occupational health, safety & regulation, Effect of radiation at molecular level, Principles and
applications of green technology, Environmental Impact Assessment: methodology, prediction and
assessment of impacts, public participation, Environmental management and ISO certification, Microwave sensing, Digital image processing and applications of remote sensing and GIS in environmental management, Radiation detectors and monitors, Judicial activism and environmental
protection, Status and distribution of wildlife in India, Environmental economy, Sustainable
development, Green economy, Environmental health, Biosphere and its evolution and major biomes in
India, Biogeography, Advances in environmental biology, Clean technology, Environment audit.
B.3 Microbiology:-
Scope and history of microbiology, Staining and microscopy, Laboratory techniques in microbiology,
Evolutionary link of prokaryotes, Morphology and ultrastructure of bacteria, Microbial growth and
reproduction, Sterilization techniques, Nutritional requirement of microbial growth, Microbial genetics,
Genome of prokaryote Eukaryote (Fungi) and viruses, Genetic elements, Extra chromosomal DNA in
bacteria and eukaryotic cells, Gene expression and regulation, Genetic recombination in bacteria,
Gene sequencing, The nature of Genetic material, replication, History and scope of recombinant DNA
technology, Cloning and expression vectors, DNA cloning strategies, Nucleic acid plotting and
hybridization, Expression of clone genes, polymerase chain reaction and site directed mutagenesis,
Nomenclature and classification of viruses, Bacterial viruses, Importance and significance of
microorganism in food, Microbial spoilage of different types of foods, Fermentation and food
preservation methods, Food safety and quality management system, Microbial growth and physiology,
Pathways and their significance, Growth kinetics and nutritional classification, Enzymes and microbial
metabolism, Bio-synthesis of macromolecules, Microbial biotechnology, Fermentation metabolism,
Fermenter/ bioreactor, Design and operation, Fermentation system, Recombinant products, Microbial
conversion and their product formation.
20 questions consisting of General Knowledge of Himachal Pradesh.
10 questions consisting of National and International affairs.

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